Palmitic and Stearic Acids: Maintaining Milk Fluidity

Posted by Dr. Jim Lofton on Aug 3, 2022 11:31:19 AM

 

The recent interest in feeding highly concentrated palmitic acid (C16:0) to lactating dairy cows for the purpose of improving milk fat test and yield has caused researchers and consultants alike to search for information to aid in making informed decisions on long chain fatty acid (LCFA) supplementation. The mammary gland is constantly combining fatty acids (FA) of different melting points to maintain milk fluidity. The melting point of C16:0, stearic acid (C18:0), and oleic acid (C18:1) is 145° F, 157° F, and 56° F respectively. The cows’ normal body temperature is 101.5° F. 200 When the mammary gland is presented with plasma triglycerides (TG) rich 150 in C16:0, the tissue blends lower melting point FA in the sn-3 position on 100 the TG which is preferentially reserved for these FA as shown in Table 1. 

Table 1. The molar % of milk fatty acids at the 3 different positions on the milk fat TG and their corresponding melting points. Adapted from Jenson, 2000.

C16:0 at high levels of intake pose a problem for the cow with respect to milk FA content. As observed in the 7 recent production trials, only 15-20% of the added C16:0 intake is incorporated into milk fat. There appears to be a mechanism in place that limits the amount of C16:0 and other high melting point FA in milk fat. Timmon and Patton 1988 reported in their landmark publication regarding milk fluidity that the small differences in milk FA, consistently detected, seem important for a number of reasons. It is notable that the fatty acids involved are crucial to the liquidity of milk fat. It is a liquid at body temperature presumably of necessity for its synthesis and secretion by the lactating cell, as well as for efficient utilization by the offspring. The principal means of assuring this liquidity is the incorporation of C18:1 (mp 56 F), which is produced from C18:0 (mp 157 F) in bovine mammary tissue, and of short chain FA (C4:0-C10:0, mp -17 to +89 F) into TGs destined for milk fat globules. The conversion of C18:0 to C18:1 in lactating bovine tissue is accomplished by the microsomal enzyme, stearyl-CoA desaturase. It is evident that the membrane fluidity-desaturase activity relationship could provide a basic control mechanism for maintaining liquidity of accumulating fat droplets.

Figure 1. Relative incorporation of acetate by exogenous fatty acids in mammary tissue in uM. Adapted from Hansen and Knudsen 1987

 

Figure 1 illustrates the stimulatory effects that palmitic and lauric acids have on denovo synthesis as measured by acetate incorporation into milk TGs. These researchers washed mammary tissue on any FA and added different FA to determine their effect on denovo synthesis. The highest melting point FA are causing increased denovo synthesis activity to accommodate need to combine lower melting point FA into milk fat TGs to maintain milk fluidity.

The composition of a LCFA supplementation appears to be very important to milk fat production and its FA composition. LCFA supplements high in C16:0 will require either adequate small and medium chain FA from denovo synthesis to balance the fluidity, or adequate C18:0 that can be readily converted into C18:1 for that purpose. In the presence of milk fat depressing situations that severely limit the small and medium chain low melting point FA availability, more C16:0 will aggravate the situation since it will be limited for use in milk fat synthesis. A balance of C16:0 and C18:0 at or near a 1:1 ratio will be superior in maximizing milk fat production.

 

References:

Loften, J. R. et al., 2014. Palmitic and Stearic Acid Utilization and Metabolism in Lactating Dairy Cows. J Dairy Sci 97

Timmen, H., and S. Patton. 1988. Milk Fat Globules: Fatty Acid Composition, Size and in vivo Regulation of Fat Liquidity. Lipids. Vol. 23. No. 7. 685-689

Jensen, R. G., 2002. The composition of bovine milk lipids: January 1995 to December 2000. J Dairy Sci. 85:295-350.

Hansen, H. O., and J. Knudsen. 1987. Effect of exogenous long-chain fatty acids on individual fatty acid synthesis by dispersed ruminant mammary gland cells. J. Dairy Sci. 70:1350-1354.

 

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Posted by Lindsey Ormond, Director of Research & Development on Jul 21, 2022 6:47:30 PM

Casein makes up 80% of the protein in milk (whey being the other 20%) and has gained more attention in recent years for its nutritional and functional benefits.

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HIGH STEARIC ACID FATS MAY REDUCE FATTY LIVER TROUBLES

Posted by Dr. Jim Lofton on Jun 9, 2022 12:55:00 PM

 

Proper nutritional management of the transition cow helps reduce the risk of fresh cow metabolic problems, increase milk production, and maximize fertility. Recent research indicates that the fatty acid profile of dietary fat may significantly affect the amount of fat that accumulates in the liver of early lactation cows.

Fat deposition occurs in the liver of all dairy cows around the time of calving. The clinical disorder, which often is termed "fatty liver syndrome”, appears when excessive fat accumulates in the liver. Outward signs in- clude a generalized failure to "come into milk," reduced immune function and increased incidence of other common post-calving diseases & dis- orders such as ketosis, poor responses to treatment for other diseases, decreased milk production, & impaired reproductive performance.

Cows are in negative energy balance during early lactation. Mobilization of body energy reserves to meet the energy needs of milk production also results in the accumulation of fat in the liver due to the liver’s limited ability to metabolize or secrete fats.

Stearic Acid Does Not Accumulate in the Liver

Recent research has shown that dietary fats high in stearic acid (C18:0) and low in palmitic (C16:0), oleic (C18:1) and linoleic (C18:2) acids may reduce liver triglyceride accumulation in early lactation cows. Stearic acid appears to be preferentially used by either the liver for oxidation (energy) and/or by the mammary gland for milk fat secretion.

Researchers demonstrated that despite serum concentrations of palmitic and stearic acids increasing to nearly identical levels postpartum, no stearic acid accumulated in the liver, even though liver concentrations of palmitic, oleic, and linoleic acids increased dramatically (four to six times in cows that developed fatty liver). The researchers observed similar changes in serum and liver fatty acid content in cows that did not develop fatty liver, but increases were of a lower magnitude (2-3x).

This work validated earlier research documenting that during fatty liver development, stearic, oleic, and palmitic acid contents increased by 70%, 860%, and 900%, respectively. Another study simulating the metabolic effects that occur in transition cows demonstrated that feeding calcium soaps of long-chain fatty acids increased liver triglyceride accumulation and reduced rate of liver fat excretion. Eight different publications now support the concept that different dietary fatty acid profiles and feeding management practices affect development of fatty liver.

Supply Extra Energy Needs Without Sacrificing Dry Matter Intake

When dry matter intake (DMI) declines or energy requirements are greater than energy intake, alternate sources of energy must be found. If dietary energy intake is not increased, the body begins mobilization of body fat stores and fatty livers can develop. Feeding an appropriate supplemental fat can lower fatty liver risk factors. When choosing a supplement for transition and high producing cows, select a fat that: 1) does not depress DMI; 2) is high in stearic acid to minimize fat accumulation in the liver; and 3) has the highest Net Energy for Lactation to meet the cow’s energy requirements.

 

  • Due to high palmitic, oleic and linoleic acid content, calcium soaps of long-chain fatty acids should be avoided in early- lactation diets as they may increase the incidence and severity of fatty liver.
  • Stearic acid does not appear to accumulate in the liver.
  • Energy Booster 100® is the only fat with a high stearic acid content and minimal palmitic, oleic and linoleic acid content that is highly digestible.
  • In addition to providing the highest NEL value, Energy Booster 100® does not depress DMI.

 

 

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RETURN COWS TO POSITIVE ENERGY BALANCE SOONER

Posted by Dr. Jim Lofton on May 11, 2022 1:39:08 PM

The critical element in how well cows fare after calving is energy balance. This in turn has several key components, namely length and extent of negative energy balance, dry matter intake (DMI), and body condition status.

Although the return to positive energy balance after calving varies considerably, the main factor in when this occurs is not milk production level, as many believe. In fact, researchers found that the time required to reach positive energy balance is independent of milk yield. Rather, the most important factor is net energy of lactation (NEL) intake, which is the product of DMI x energy density of the diet.

Since it is difficult to increase DMI in early lactation, the component to alter is energy, as long as it does not reduce DMI. When calcium soaps of fatty acids (CSFA) are used to increase energy density, intake is reduced, which results in more time to reach positive energy balance as seen in Figure 1 for first-calf heifers and in Figure 2 for older cows. The differences in intake between the Energy Booster 100 (EB-100) and the CSFA ration are based on NRC 2001,7 which established that a 1% inclusion of CSFA reduced DMI by 2.5%. This difference translates into heifers and cows fed an EB-100 ration achieving positive energy balance 30 days sooner than when fed CSFA.

Figure 1.

Figure 2. 

Loss of body condition and the corresponding rising non-esterified fatty acid (NEFA) levels are further evidence of CSFA’s negative effect on energy balance. The best physiological indicator of energy balance is plasma NEFA levels. The more body condition mobilized, the greater the NEFA level. NEFA levels have been shown to increase as dietary CSFA levels increase,2 to be greater in mid-lactation first-calf heifers averaging 80 pounds daily milk production when fed CSFA vs. EB-100,6 and to be greater in mid-lactation older cows averaging 93 pounds daily milk production when fed CSFA vs. EB-100.4

So how does lower NEL intake due to decreased DMI affect productivity? Using NRC 2001 NEL values to calculate the effect of energy intake loss on milk yield, inclusion of CSFA trans- lates to reduced milk yield of 5 to 8.5 lb per day, which totals 1,100 lb over the first 140 DIM. If the reduced energy intake is instead converted into body weight loss, the range of daily loss ranges from about 0.75 to 1.33 lb, or a total of 176 lb over the first 140 DIM. In reality, some combination of both milk and body weight loss likely will occur. This energy deficit also negatively impacts reproduction because energy balance during the first 3 to 4 weeks postpartum is correlated with interval to first postpartum ovulation.

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Topics: ANIMAL NUTRITION

THE IMPORTANCE OF NET ENERGY INTAKE AND REPRODUCTIVE PERFORMANCE

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